Study of the immunoglobulin and oxidized protein content of semen under infertility

Among the causes of male infertility, enough attention is paid to oxidative stress, which in turn a pathogenetic link inflammatory process. However, there practically no information on content oxidized modified proteins semen, makes it difficult study pathogenesis diseases reproductive system. In part, protein oxidation may be due production reactive oxygen species by microorganisms, both directly and indirectly through activation immune system cells. The aim research was level changes immunoglobulin concentrations semen under bacteriospermia. A made ejaculate 48 men who applied clinic for infertility marriage. comparison group consisted 32 healthy had growth microorganisms samples. When conducting bacteriological analysis, studied samples were diluted 10 times used generally accepted method. concentration albumin, immunoglobulins A, M, G, E determined spermatic fluid. modification evaluated reaction with 2,4-dinitrophenylhydrazine. expressed nmol/mg total biological biuret method determine concentration. Statistical analysis results performed using descriptive statistics Student's t-test paired data. seminal fluid did not differ significantly among groups. albumin (16.96±1.28 mg/mL) statistically lower absence microorganism than With bacteriospermia, decrease IgM IgA an increase IgG noted. degree maximum when enterobacteria are isolated from Thus, during studies found that, despite clinic, asymptomatic secretion G into observed. accumulation bacteriospermia has been shown.